02287nas a2200277   4500000000100000000000100001008004100002260007000043653003900113653001000152653003200162653002400194100001200218700002200230700001300252700001200265700001300277700001500290700001000305245011500315250000800430300001200438490000800450520153700458022001401995       2017                            d  c03/2017bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10aCorynebacterium pseudotuberculosis10aELISA10arecombinant phospholipase D10awhole-cell antigens1 aR Sting1 aL Schneider-Bühl1 aH Wagner1 aJ Maget1 aB Polley1 aD Bürstel1 aH Axt00aClinical and serological investigations on caseous lymphadenitis in goat breeding herds  in Baden-Wuerttemberg  a3/4  a133-1430 v1303 aData on clinical and serological examinations were collected within the first step of a programme for control of caseous lymphadenitis in goat breeding herds in Baden-Wuerttemberg, a federal state of Germany. For the serological studies, an in-lab ELISA based on whole-cell antigens and a commercially available ELISA based on recombinant phospholipase D were evaluated, revealing sensitivity values of 85%−91% and specificity values of 93%−100%, respectively, depending on the cut-off value defined. Comparative evaluations on the agreement between the results of both ELISAs reached kappa values of nearly 0.9 and a proportion of agreement of about 95%. A total of 1333 goats from 27 herds were examined clinically and blood samples were taken from each animal for serological testing. Using the in-lab and the commercially available ELISA test, 5.3% (n = 71) and 4.8% (n = 64) of animals originating from nine and five herds revealed positive results, respectively. In clinical examinations, 5.2% (n = 69) of goats from 12 different herds displayed palpable swelling of lymph nodes, particularly affecting the Ln. cervicalis superficialis (n = 30), followed by the Ln. mandibularis (n = 11), Ln. inguinalis superficialis (n = 9), Ln. popliteus (n = 9), Ln. parotideus (n = 5), and the Ln. retropharyngeus (n = 2). Results of clinical positive and serological positive animals differed. However, clinically apparent goats revealed a significant higher rate of serologically positive results than clinically unapparent animals.  a0005-9333